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XI Международный Научный Конгресс "НАУКА. ИНФОРМАЦИЯ. СОЗНАНИЕ"

7-9 июля 2007 года, Санкт-Петербург, Россия

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PART 5. PRINCIPLES OF EPC ANALYSIS OF LIQUIDS AND MATERIALS


Water as test subject

The 19th century science focused on exploration of the ideal systems in a state of rest or in a state of simple motions. The 20th century science has significantly extended horizons of world understanding, a qualitative transition to description of probabilistic dynamic processes has taken place, but, generally speaking, this description has resulted in exploration of capture of the running processes, at best case with consideration of simultaneous interaction of two or three reagents. It allowed creating beautiful models of the world around us but these models were static and single-step. As any models they turned out to be limited, ideal and describing just separate sides of reality. But, as it always happened in the history of science, these models were apprehended as adequate reflection of the external reality. There emerged an impression that a modern scientific paradigm described the world around us completely. Understanding of complexity, dynamics, and multi-compoundness of the proceeding processes practically fell out of consideration. A picture of lineal, logic, and predictable world was created where each action leads to a certain situation.  
The principles of the modern classic medicine are based upon such paradigm.  Intake of a certain medicine produces a well-known response. All organism processes are based on chemical interactions, and control under them allows influencing the state predictably. An organism’s features are preset by genes, rearrangement of which allows controlling past, present and future. We understand peculiarities of processes in a molecular level, we can control these processes and the surgeons can replace the worn organs, if necessary, and the patient with a new body starts a new turn of his life. Just a little ahead and it will be possible simply to grow a body in autoclave and deliver it to a client instead of the worn one.
 Though, such ideas have proved to become a great illusion. More than a hundred years of western medicine development have revealed inability to cope with major diseases, in spite of tremendous efforts and means invested in this field. Cardiovascular, oncological and chronic diseases have come to take place of the infectious and acute disorders. All talks about cloning and genetic modification are the investors’ fraud with the purpose of pumping out money and often are just rigging of the results. The problem is that all modern biologic concepts are based on the idea of linear logic world.
Simple straight mentality was applied when carrying out water and water systems research as well. Chemical formula was defined, basic properties were revealed and composition of life-useful and life-dangerous admixtures was determined. That was the point at which the debates started as it became unclear - what was the use of the microelements? Probably, it was better to drink super-cleaned distilled water and not to think about the source from which it had been extracted. Such point of view was accepted in a number of countries but afterwards distillate was found not as useful because it washed out microelements, bones became fragile and hair became brittle. So, they began to add salts to distillate and declared that the obtained water was useful for life. Here again they started talking about something missing in this water and that real natural water was still ever better. But how could it be proved? What about the competent word of science?
Obviously, here again we face the fact that Nature is not so simple and predictable as it seems to us. Especially, as far as human health and, particularly, the foodstuffs is concerned.  One may remember enthusiastic welcome of hydroponics, i.e. growing of vegetables and fruit in water solution. No more problems of vegetable gardens, fertilizers, bugs and other pests. Closed factories are holding lines of plastic containers through which solution is pumped, in which red tomatoes and round apples ripen under the rays of artificial sun. Here is the civilization’s triumph over nature! But soon these tomatoes and apples turned out to be tasteless and odourless looking more like plastic toys than foodstuff. Just try to smell fruit and vegetables from the marketplace and you’ll feel the difference very soon.
The same is with water. Try to carry out a simple test: water your flowers first with natural and then with artificial water. You’ll see the difference in a week. Fish react to water composition more acutely but it’s pitiable to take tests on them. Still some scientific means of research are desirable. So, water test seems to be not an easy matter at all.
One of the basic suppositions of classic physics states that the test subject remains invariable if certain conditions are maintained, so it is possible to obtain the same results in different laboratories and during different daytime and season. Classic physics and chemistry base upon it. As soon as we start testing water we become ascertained that its properties are subject to change in the most unpredictable manner. Naturally, the question is in slight variations regards the basic parameters but exactly this is interesting for the modern researchers.
Let us take a hermetically sealed bottle of distilled water, open it and pour water into several vials of glass and plastic. Let us measure the initial parameters: pH, specific conductance, quantity of dissolved oxygen, total electrolytes concentration, super-weak liquid glow stimulated by electric field (EPC) or chemically (biophotons). Let us leave these vials for a day and then repeat measurements. We'll become assured that the parameters in the different vials are different. We’ll obtain the major difference for the plastic vials, especially if they were filled with water not to the top or were left uncovered in the open air. And for the glass vial difference of parameters will be quite significant. This simple experiment demonstrates that water parameters depend on certain factors and change dynamically in the course of time. It is possible to place emphasis to the main factors affecting water parameters:
­    interaction with air gases;
­    absorption of dust from the air;
­    impact of light, especially of sunlight;
­    interaction of water and vials walls, especially with plastic;
­    formation of water layers around molecules of admixtures;
­    gas emission from water itself, oxygen and hydrogen mostly.
These processes are schematically shown in the picture. Naturally, they significantly depend on temperature and pressure of surrounding air.
In order to minimize impact of these factors the researchers usually strictly control all conditions and, what is the most important, in their tests they use bi-distillate totally purified of any admixtures. Naturally, the obtained results are of great significance but they do not allow saying anything about properties of water surrounding us in our everyday life as this water contains different admixtures, which cardinally change its behaviour.
The molecules of admixtures are the clustering centres and structure of these clusters corresponds to architecture of the molecules of substance. Water molecules seem to repeat configuration of the introduced substance and the formed clusters have stable structure preserved during long time. More and more evidences are accumulated, which prove that influence of electromagnetic fields on water and "water memory” effects are connected with cluster formation. Let us examine separate cases of these effects.
Water as EPC test subject

Recent years are remarkable for increased attention to study of water structural properties and possibilities of information transfer through water. There is a mature standpoint that experimentally observed phenomena are caused by the processes of clusters and clathrates  formation mainly in the atoms of admixtures. In order to introduce these concepts into context of modern scientific thinking primo needed is a set of evidential and reproducible experimental factors. Complexity of water as a test subject and dependence of its properties on numerous factors lead to necessity of simultaneous use of several independent methods as well as to necessity to develop and implement new informative methods of water properties research.  
Multiannual researches taken in different countries have demonstrated that EPC method is very sensitive if applied for studying substances and material properties. Its performance capabilities have proved to be unique in many fields. Six patents and scores of publications are the evidence of it.
Informativity of EPC method for studying liquid-phase subjects has been demonstrated during research of glow of microbiological cultures  [Gudakova G., et.al. 1990], blood of healthy people and cancer patients [Korotkov K.G., Gurvits B.Y., Krylov B.A. 1998], blood response to allergens [Sviridov L.P.,et.al., 2003], comparison of natural and synthetic, organic and ordinary, clockwise and counterclockwise rotating samples of grammatical oils [Korotkov, Krizhanovsky et al, 2004], homoeopathic preparations of 30C potency [Bell I., et.al. 2003] and flowers essences [Korotkov K. 2003], ultra-low concentration of different salts [Korotkov K. et.al. 2004],. Recently the unique data of biologic activity of hair have been obtained [Vainshelboim et al, 2004].
In particular, it has been demonstrated that samplings of EPC images parameters of distilled water taken in different days do not show any statistically significant differences. The same results have been obtained for one-normal solution of electrolytes NaCl, KCl, NaNO3 and KNO3 that allows making a conclusion that the liquid-phase subjects data show high reproducibility with EPC-graphy method. Difference of solutions and distilled water glow parameters stays up to 2-15 dilution, though dynamic trends of 2-15 dilution and distilled water show different directions in this case as well.
The works on revealing differences in glow of natural and synthetic essential oils of the same composition have aroused great interest.
The oils were tested in respect of possibility to reveal differences subject to natural and synthetic method of their production, as well as oils of organic and regular origin; oils produced in different climate conditions and extracted by different methods; oils of different optical activity; fresh oils and oils oxidized by different means. The tested oils combinations did not have statistically significant differences when analyzed by means of gas chromatography method.














Fig.1.21. Time dependence of intensity of EPC image of Bitter Almond oil and its synthetic analog Benzsldehyde.

Testing the differences of natural and synthetic oils revealed that main differences declare themselves by high intensity value and lower value of flare area of the natural oils. Differences may become apparent in a definite time interval after beginning of observation of EPC processes for oils (fig. 1.21). For example, oils of Russian, Bulgarian and Moroccan rose demonstrate characteristic dynamics of the times series trends, at which during first 0.06 seconds these oils showed no significant differences, and at the same time on expiration of this time statistically significant differences started to appear between the Russian rose oil and of the other two oils. Statistically significant differences between Bulgarian and Moroccan oils appear after 0.9 seconds of observation. Study of oils with different optic activity is of a special interest. This group of oils belongs to stereoisomers, i.e. compounds built of identical set of atoms with the same sequence of chemical bonds but differing in arrangement of atoms in tridimensional space. Interacting with these media beam of light becomes clockwise or counterclockwise polarized. The results of the experiment reveal that pairs of oils Dextro Carvone v.s. Laevo Carvone, Dextro Limonene v.s. Laevo Limonene and Dextro Linalool v.s. Laevo Linalool show different parameters of EPC images. In cases when fractal dimension of counterclockwise rotating media (Laevo Limonene, Laevo Linalool) is less than that of the clockwise rotating isomers, increase of time series trends of flash area is observed. In case of Dextro Carvone v.s. Laevo Carvone, counterclockwise rotating oil media Laevo Carvone has bigger fractal dimension and time series trends of flash areas are decreasing. During study of 60 pairs of oils with similar chemical composition statistically significant differences by different analysis methods are observed with 52 combinations of oils.
 Below please find the results of one of the tests of water glow study.

Contents of work:
The test subject is bottled portable mineral water bought through St.Petersburg trading network, marked as W1, the same water with bioactive agents (BAA), (sampling W2). The liquids have been tested immediately after opening the packing and in four hours after opening (samples W3 and W4 correspondingly). Pharmaceutical distilled water with salt admixtures in ampoules served as control sample.
During testing EPC parameters of liquids a drop is suspended 2-3 mm over glass surface of optical window of the device and glow of liquid meniscus is registered. Time dynamics of EPC parameters has been measured by means of the commercial device EPC Camera produced by KTI, St.Petersburg (www.kti.spb.ru). In order to evaluate data statistic reproducibility at least ten independent measurements for each water type have been taken and the results have been averaged.
All tests are taken under temperature range of 22.5-23.5С and relative humidity of 42-44%.
The test results:
Fig. 1.22 shows diagrams of EPC parameters change in dependence on exposure time of solution drop to electric field.
 
Fig. 1.22.  Time exposure of EPC glow area of a water drop.
1, 2  - Samples W1 and W2 taken immediately after opening a bottle.
3, 4  - Samples W1 and W2 taken in four hours after opening a bottle.
5 – distilled water with salt admixtures.

As the given data show glow of water captured after immediate opening a bottle reveals higher variability of measurements and significant increase of parameters with two pronounced phases: during the first 30-40 seconds and further up to two minutes; the results become stabilized in two minutes. For water samples exposed to open air during four hours increase is observed during first 40 seconds, then the parameters are stable. The same behaviour is typical for salt solutions though their glow amplitude is significantly lower. Several conclusions can be made basing on the obtained data.
1. Immediately after opening the mineral water interacts with atmospheric oxygen and with applied field, and changes its state actively until stable level is achieved. Obviously, this process is similar to aging of wines in the open air.
2. A process of active liquid structuring with increase of glow amplitude takes place within first 30-40 seconds of field application. This process might be connected with formation of conductivity channels in liquids.
3. When liquids are kept in the open air within four hours the amplitude and character of glow of liquids significantly changes. This might be connected with decontamination of mineral water.
4. BAA addition haven't influenced character of its glow.
Conclusion
The given data demonstrate that EPC method has high selectiveness and sensitivity if applied to study of liquid-phase subjects and different types of water in particular. The information being obtained depends on chemical composition of water but determinative and most interesting is the dependence on structural composition of liquid. EPC glow parameters are determined by emissive activity of liquid surface layer, which depends on availability of surface-active valences. Obviously, this feature is determined by structure of the near-surface clusters, i.e. EPC method is one of the informative methods of structural and informational liquid properties research. At present time there is every reason to include EPC method into the set of integrated tests of liquid and water properties.
Study of human consciousness impact on water EPC parameters is an interesting research trend. Numerous tests revealed that mental impact leads to statistically significant alterations of EPC glow of water and these alterations are preserved during long period of time. These results have not only gnoseological but a pure practical meaning as they prove that quality of food depends on mental tune of the people cooking this food. As they say, “better to take poison from  wiseman’s hands that manna from enemy’s hands”. We are sure that EPC method will find more numerous applications in liquid properties studies.

Application of the GDV-graphy technique for the estimation of antigen-antibody reaction

Stepanov A, Sviridov L, Korotkina SA, Achmeteli GG, Kriganivski EV.
Research Center, St. Petersburg, Russia

    At the present moment the spheres of application of Gas Discharge Visualization (GDV) technique in biology and medicine are widely searched. Basing on the well-known fact that this technique can detect changes of physico-chemical characteristics of solutions of non-organic substances and biological liquids, we made an attempt to study the possibility of application of gas discharge glow for the registration of specific interaction of an antigen with a complimentary antibody – the so-called agglutination reaction. This very fact defined the aim of the present investigation.
    In order to realize this aim the authors developed and applied the method of estimation of the characteristics of gas discharge glow around a drop of liquid. The method assumed that the drop of liquid should be pressed out of a disposable insulin syringe and placed in its end. Each sample was tested 10 times with the frequency of 30 shots a second and the duration of influence of the electromagnetic field – 10 seconds. Summary data of the GDV tests were compared with the results obtained by standard visual method.
    Specific antibodies were received by the way of immunization of rabbits by ovalbumen or tularemic vaccine and the white mice – by specific vaccine. Apart from that the blood serum of a person recovered from the infection caused by conditionally-pathogenic bacteroid B.fragilis was used.
    In order to carry out the main experiments it was first of all necessary to optimize the scheme of GDV analysis. Particularly, the following question had to be solved: was it necessary to stir the antigen-antibody complex laid-down on the bottom of the test tube? This part of the work was performed using the following complementary pairs of reagents:
•    Ovalbumen + antibodies (blood serum of rabbits immunized by albumen);
•    Vaccine strain of the specific virus+ antibodies (immunoglobulin against virus, obtained from ascetic liquid of white mice, inoculated with the corresponding vaccine);
•    Tularemic antibody + antibodies (blood serum of rabbits inoculated with tularemic vaccine).
Using the abovementioned systems similar data were received. It turned out that even when the sediment (agglutinate) was carefully stirred the results of GDV analysis didn’t agree with those of the visual test; moreover, they often contradicted the latter.
In case of GDV investigation of the supernatant when the sediment wasn’t previously stirred, the results coincided with the data of visual registration of agglutination reaction. We suppose that under positive reaction the antigen-antibody complex formed sediment and the supernatant “got cleaned” from the components of reaction. When agglutination was not performed, the components of reaction were found in the suspension state, i.e. no cleaning (clarification) of the supernatant took place. The GDV technique registered visible (clarification of supernatant) and invisible (change of physico-chemical properties) differences in experimental and control (known negative) samples appearing at that; thus the technique indicated positive agglutination reaction.
Considering these data detailed experiments proving this generally formulated aspect were carried out further. One of these experiments was performed with the application of the following reacting components:
1.    Human blood serum with antibodies to B.fragilis in titer 1:640 (serum N 1);
2.    Human blood serum without B.fragilis antibodies (serum N 2);
3.    Suspension В.fragilis (antigen);
4.    Physiological solution (0,85 % solution NaCl).
The scheme of experiment is shown in table 1.
Table 1. Scheme of experiment
1*
0,85 % NaCl    2
0,85 % NaCl + B.fragilis    3
0,85 % NaCl + antibodies to B.fragilis    Interspecific (complimentary) components of reaction     Combination of components in 24 h before
 investigation    4
0,85 % NaCl+В.fragilis+blood serum N1    5
0,85% NaCl+B.fragilis+blood serum N1 in dilution 1:1000
                Combination of components right before
 investigation    6
same    7
same
            Internonspecific (non-complimentary) components of reaction     Combination of components in 24 h before
 investigation    8
0,85 % NaCl+В.fragilis+blood serum N2 in dilution 1:10    9
0,85 % NaCl+В.fragilis+
blood plasma N2 in dilution 1:1000
Comment: * - shows the number of a sample.

Investigation of samples 1,2 and 3 (physiological solution NaCl, the same solution with B.fragilis or blood serum N1, respectively) was aimed at determining if the GDV technique could disclose such microscopic objects as antibodies and microorganisms in the solution.
Comparative assessment of GDV-grams of these three objects determined that sample 1 reliably differed from sample 2 in the intensity of glow and in background area, and from sample 3 – not only in these criteria, but also in the form coefficient and length of isoline. Samples 2 and 3 reliably differed from one another in all the abovementioned parameters. To our mind, all that indicates that the applied method enables to disclose if such biological objects as microorganisms, being the model of antigen in this case, and immune serum, containing specific antibodies to these microorganisms, are present in physiological solution. Moreover, differences between the blood serum and suspension of microorganisms can be revealed.
The results of assessment of samples 4-9 should have answered the main question: could the GDV technique identify not only antigen or antibodies to it in the physiological solution, but also specific reaction between them – agglutination reaction? This is possible theoretically, as new biological structures (immune complexes) are formed at such interaction. These structures possess different physico-chemical properties as compared to other initial reagents (antigens and antibodies), and most probably change the characteristics of the solution.
The main (pilot) sample of all was sample 4, which consisted of human blood serum with antibodies to B.fragilis, diluted by physiological solution up to titer 1:10, and suspension B.fragilis. Agglutination reaction was inevitable in such a system in view of specificity of the biological reagents taken, which was visually proved in 24 hours (when GDV-grams were taken).
Control sample 5 differed from sample 4 only by the fact that it had blood serum diluted at 1:1000. In this case no agglutination reaction took place, as the serum dilution (1:1000) exceeded its maximal titer (1:640).
Comparing GDV-grams of samples 4 and 5 it was found that they significantly differ in the intensity of glow. The only difference between them consisted in the fact that agglutination reaction took place in one sample (sample 4), and was absent in the other (sample 5), which fact proved that the GDV technique could register new physico-chemical state of sample 4, which took place as a result of specific interaction of antigen and antibodies in it. In other words, the technique could visualize the agglutination reaction.
The following aspects prove this conclusion, as well:
- reliable differences in the intensity of glow (fig.1.23) between samples 4 and 6 (there was no agglutination in sample 6 as a result of insufficient exposure within the mixture of components: the components had been mixed right before the GDV-grams were taken, i.e. agglutination reaction hadn’t yet taken place);
 - reliable differences in the background area (fig.1.24), intensity of glow (fig. 1.25) and length of isolines (fig.1.26) between the samples 4 and 8 (agglutination in sample 8 didn’t take place because of the fact that there was no specific antibodies to B.fragilis in blood serum N2);
- absence of differences between samples 6 and 7 (agglutination was absent in both samples because of insufficient exposure of antigen with antibodies);
- absence of differences between samples 8 and 9 (agglutination didn’t take place in both samples as a result of heterologicality of antigen and antibodies).
Thus, as follows from the data above, comparative analysis of GDV-grams of pilot and control samples enables to reveal antibodies in the investigated material.
It was also considered important to study the informativeness of the GDV as a method of titration of immune sera. Special experiment on the determination of titer of antibodies in the blood serum of rabbits, inoculated with ovalbumen, was planned for that. The scheme of the experiment is represented in table 2.

Table 2. Results of titration of the blood serum of rabbits, inoculated with ovalbumen
NN of samples    1    2    3    4
Dilution
of serum    1:10    1:20    1:40    1:4000
Titer of antibodies according to the data of visual assessment    ++++    +++    +    -

Comparative analysis demonstrated that the GDV-grams of samples 1 and 2, similar in visually registered agglutination (4+ and 3+ respectively), didn’t differ reliably. At the same time, sample 1 reliably differed in area and intensiveness of glow from sample 3 (agglutination +), and in area, intensity of glow, and also in the number of fragments (fig. 1.27) from sample 4, where agglutination was absent at all. Analogous differences were found comparing GDV-grams of sample 2 with the characteristics of sample 3 and 4. At the same time, computer analysis of samples 3 and 4, where agglutination was expressed weakly (sample 3) or was absent at all (sample 4), didn’t reveal differences between them.
Therefore, the titer of antibodies determined by visual and GDV methods turned out to be the same: 1:20. This fact forms the basis that the GDV technique gives an opportunity not only to register antibodies in liquids, but also to determine their titer.
CONCLUSION
Basing on the presented materials we can conclude that the GDV technique enables to identify specific reaction of antibodies with a complimentary antigen, called agglutination reaction. The technique is based on the registration of dynamics of parameters of gas discharge glow in time – from the moment of combination (mixing) of specific components (antigen and antibodies to it) to the moment of completion of their interaction and formation of the so-called immune complexes. As a results of such interaction, physico-chemical characteristics of the investigated material, and consequently, GDV-gram parameters change.
The results obtained are of course preliminary and need to be specified further. But already now we realize the significance of this direction of scientific search.
The technique can be applied for the investigation of nontransparent biological liquids when it is not only difficult, but even impossible to implement the agglutination reaction in its classical form (visual registration of results); for example, researching the blood with the purpose of revealing etiology of human allergies.

 
Fig.1.23. Time dynamics of glow Intensity of samples 4 and 6.
 
Fig.1.24. Time dynamics of glow Area of samples 4 and 8.
 
Fig.1.25. Time dynamics of glow Intensity of samples 4 and 8.
 
Fig. 1.26. Time dynamics of glow length of isolines of samples 4 and 8.
 
Fig.1.27. Time dynamics of glow number of fragments of samples 3 and 4.
 
Features of EPC substances analysis

Please read the instruction to EPC Mini Laboratories and description of methods carefully before commencement of work.
There were cases when people tried to perform research of substances without sufficient operation experience and obtained absurd data or simply couldn’t fix EPC images, for example, of water.

Work with substances is recommended only after mastering all EPC programs and methods.

KTI Company conducts dedicated seminars and trainings to prepare certified specialists. EPC research of substances could cause problems without receiving training at these seminars.

All EPC tests of substances are taken in dynamic mode.

EPC analysis of substances is divided into three main stages:
1)    Registration of BMP or AVI files of samples glow;
2)    Data processing in the program EPC Sci Lab;
3)    Analysis of the obtained results with possibility of their further processing;
4)    Formation and preparation of the report and publications.

At the stage (1) in order to obtain data statistic authenticity it is necessary to make at least 6 captures of image of one sample.
Interval between capturing images is from 10-15 seconds to 1-3 hours depending on the research tasks.
The more time the capture takes the bigger the size of AVI file and the longer time it takes to process the file, though in this case the information about behaviour dynamics of the test subject is more complete.

Depending on the research tasks the standard size of images (320*240) or capture of small images (160*120) are used. Here size of AVI files is smaller, time dynamics of curves behaviours remains unchanged, though part of information about EPC grams structural features could be lost.

Before each capture it is recommended to carry out calibration and thoroughly wipe the optical window of the device.

At the stage (2) AVI files are processed in EPC Scientific Laboratory software. This software is especially dedicated to carry out research works and is remarkable for many useful features. All operations included in this software could be performed by other programs, such as Statistica or Mathcad, though it takes incomparably more time and efforts.
Processed results depend on chosen parameters of noise.
Choice of processing parameters depends on research tasks. The more parameters are used the more time processing takes.
Saved diagrams could be used for analysis and results presentation though they often require perfection to present the materials to the magazines.
Saved table of results can be loaded to any standard static program and serve as a base for further analysis.
The data files obtained by EPC Scientific Laboratory can be processed by program such as Excel or Statistica. It allows comparing different data, create diagrams, and use additional methods of static analysis.
Very interesting application of EPC water study is study of the influence of human intentions to water.

Measuring of mental intentional influence to water samples with EPC/GDV technique

INTRODUCTION
The aim of the study was to investigate the possible remote mental influence of a healer on water samples from different distances and in different modes by using Gas Discharge Visualization (GDV) technique. Experiments were performed in several independent sessions during two years.

EXPERIMENTAL DESIGN
GDV Camera instrument produced by “Kirlionics Technologies International”, St. Petersburg, Russia (www.korotkov.org) and used in the experiments had the following parameters: single impulse duration 10 mcs; repetition frequency 1000 Hz; induction interval 0,5-10 s; electrode voltage 3-15 kV.
1 ml of commercially available drinking water from the sealed before the experiment bottle was placed in standard syringe, which was installed into a special device of the GDV Camera that allowed a drop of liquid to be suspended 3 mm above the lens of the Camera. 5 subsequent GDV images and a short 2-10 sec film (AVI file) were taken. After that both the device and the GDV Camera stayed intact. A person or a group of people situated not less than 1 meter from the device was asked to meditate trying to influence the water in the syringe by sending “love message” to the water. After 10 min of the intentional concentration the measurements were repeated.
Processing of data was done using a set of GDV programs [Korotkov K., 2002].

EXPERIMENTAL DATA

ST. PETERSBURG, RUSSIA, CHRISTOS DROSSINAKIS, JANUARY 2002.
The mental influence to water was performed several times during Drossinakis’ visit to St. Petersburg. Healer was seating several meters from the GDV camera with water installation (see picture 1).
Fig. 1 demonstrates diagrams of water drops GDV-gram area averaged over 5 samples for the initial water (outer circle) and water after treatment (inner circle). The diagram indicates significant statistical difference between the initial water and water after treatment. As we can see from this diagram, after the treatment energy level of water decreased.
Significant difference was also found in various GDV parameters between the GDV-grams of initial water and water after treatment. Fig.2 demonstrates examples of two parameters: brightness and BEalpha. As it is shown by the graphs, both the brightness and BEalpha increased after the treatment.
 
Fig. 3 demonstrates examples of the GDV glow area dynamic behavior for initial and treated water. As we see from the graphs the type of dynamics is absolutely different: there are clear differences between the initial water and water after treatment. For the initial water dynamical behavior is quite typical for the normal drinking water: it has exponentially increasing behavior to some saturation level. The type of curve is quite reproducible, but absolute values may differ from sample to sample. After the remote treatment we see the absence of exponential grows and strong fluctuations.
Experiments were repeated three times with similar results.


GERMANY, JAPAN - TO RUSSIA. CHRISTOS DROSSINAKIS, APRIL 2002
In agreed days five plastic 1-liter bottles of tap water were placed at 10 a.m. at the table in the experimental room of the University known to Drossinakis from his visit. Every bottle was labeled with color strip. Bottles were left intact till 4-5 p.m. when GDV measurements of water samples from every bottle were undertaken.
Christos Drossinakis performed mental influence within four days (from eight agreed days) at 12 a.m. for 10 min to two bottles from Japan and Germany. Researchers performing measurements were unaware neither of the influence performed no of the target bottle. So the second experimental session was organized in accordance with twin blind study design.
In this second session of distant remote influence significant changes in GDV parameters of water drops between samples from different bottles were found only in two days. This difference was statistically significant and reproducible in successive measurements. It was days of Eng. Drossinakis’ influence from Japan. In other six days no significant reproducible difference between samples was found.
Fig. 4 demonstrates dynamical curves measured on April 09, 2002. As we see from the graphs, behavior of curves from the bottles NN 1 and 2 are clearly different from three other samples: curve N 1 exponentially increases in time, and curve N 2 has much higher level of variation compared with other samples. It should be noted that these variations were persistent, but irreproducible from measurement to measurement.  
Fig. 5 demonstrates dynamical curves measured on April 10, 2002. In this case curves for samples NN 4 and 5 have different behavior compared with other samples. For sample N 5 subsequent measurements revealed interesting behavior (fig. 6):  at the first measurement the curve had a strong variation, while the same sample, measured 10 min after, demonstrated behavior, very similar to the curve of sample N 4 from fig. 5. This type of curve was repeated in all other subsequent measurements (fig. 6, curve 3) and after the third measurement the curve became quite reproducible. It should be noted that this type of curve is quite different from all previously measured samples (fig. 3,4).

BASEL. SWITZERLAND, ALEXEY NIKITIN, NOVEMBER 2002
 
Influence was performed from 2 m distance (see picture 2). As we see from the graphs of Fig.7, the influence was quite significant. Lines 1-3 represent repeatable measurements of the initial intact sample. After the first influence the difference is clear (line 4), but it becomes significant after the second influence (line 5). We pay attention that the character of time dynamics changes.
In another session transformation was even more dramatic. Fig.8A presents several measurements of the initial sample. After the influence we see strong variations for the Form Coefficient (fig.8B) and Fractality (fig.9) parameters.
DRESDEN, GERMANY, VIKTOR PHILIPPI, MARCH 2002
Different type of influence was demonstrated several times by the well-known German healer Viktor Philippi (fig.10). After his remote influence dispersion of water time dynamics strongly decreased, but after some time it came to the initial level. This type of influence was repeated for several times.
WASHINGTON D.C., GROUP INFLUENCE,  MARCH 2003
A group of people was offered a simple test: meditation with intentional influence to water. Collective meditation lasted for approximately 5 minutes. After this the measurement was performed. Practically no changes were recorded.
Then Professor W. Tiller suggested repeating the meditation. Participants meditated for about 5 min. Measurement done just after this allowed to record statistically significant changes for the most of GDV parameters (fig.11) (p < 0.001).
After this W. Tiller suggested to change the mode of the meditation. Participants meditated again for about 5 min. GDV parameters of water measured after this were practically identical to the previous measurement after the second meditation, but the amplitude of their Fourier transform different significally (fig.11).
Water may be sensitive to different types of influence, not human only. As an example we present results of two experiments.

ARIZONA. APRIL 6, 2003
Experiments were held at the GDV workshop at Mayo Clinic in Phoenix on April 6, 2003.
A sample of commercially available drinking water from the plastic bottle marked “Arrow” was tested with the GDV Camera. Dynamical mode for 10 seconds with a drop of water suspended 3 mm above the optical lens was used. Measurements were repeated three times with new samples taken from the bottle. Good repeatability of data was found (fig.12). Then water from the bottle was poured to two glasses. One glass was placed on the top of a flower picture claimed to have some special influence. Another glass was placed at the top of the sample of the Ammonite Shell. Glasses were positions at the table 6 feet apart and left intact for 30 minutes.
After this samples of water were measured at the GDV Camera. Significant changes for the most GDV parameters were found (fig.12). The only exclusion was the GDV Form Coefficient, where no changes were found.
Then the water from the bottle was poured to two glasses again. Two participants tried to imprint intentions to the water keeping hand one foot above the surface of the water for 1 minute. After 10 minutes the samples were measured with GDV.
Significant changes for the following GDV parameters were found: Intensity, RMS and Entropy.

ST. PETERSBURG, MERUS RINGS
Special rings are produced in Germany by MERUS company for purifiing the water. Their influence was tested in a series of experiments.
Water from the bottle was poured into the glass vial and vial was positiond on the tested ring for 15 min. After this water from the glass was measured 5 times. Then measurement was repeated with 2 other rings. Fig.13 demonstrates dynamical curves of GDV area for different samples. We can see statistically significant differnece between control water sample (curve 1) and other samples (curves 2-5). As we see from these graphs, the control sample demonstrate exponentially ascending curve. This is typical reproducible type of curve. After exposing water to tested rings two different types of reactions from different rings were measured: negative inclination at the first seconds of measurements, the most typical (see curves 2-4) and after the influence of some rings time dynamics was ascending (curve 5). The difference between control sample and influence is even more clearly presented by the GDV parameter intensity that represents averaged intensity of pixels (fig.14). At the same time between the influences of different rings no difference was found.

UKRAINE, HUMAN INTENTIONS
Experiments as described above may be conducted by any GDV user. As an example we present graphs sent from MADRA center, Dnipropetrovs’k, Ukraine. This center is led by Dr. E. Semenichin. A sample of drinking water was divided to several vials. To one a drop of eucalyptus oil was added, two others were influenced by healers from 1 m (3 feet) distance. As we see from the graphs (fig.15) intentional human influence was stronger than the impact of oil added to water.

CHANGE OF WATER PROPERTIES BY SLOVENIA GLASSES

Three glasses of the same volume were filled with the same amount of drinking water: standard glass, Slovenian glass with blue mark, Slovenian glass with green mark. Glasses were placed at the distance more than two meters from each other. After 30 minutes samples of water from every glass were measured in different regimes. As could be seen from the graphs of fig.16, there were statistical difference between samples. From the type of changes we can make a conclusion that Slovenian glasses increase structurisation of water.
Experiment was repeated five times with similar results.
It was also noted that when all three glasses were standing nearby, parameters of water stimulated emission have been changing in all glasses synchronically. The impression was that Slovenian glasses were influencing the neutral glass. But this observation needs more careful study.

CONCLUSION
All given data may be considered only as preliminary. These are rather observations than strict experiments. The development of the protocol of a randomized blind experiment with strict control of all conditions is required. In this experiment it is necessary, first of all, to test experimentally the stability of the GDV water parameters in time without the intentional effect. Then, we need to have control test samples measured in parallel to the effected sample. Finally, we need to take into account and study the space conditioning effects considered in W. Tiller work [2001]. Such work should be the subject of a separate research which needs certain support.
At the same time the obtained results, taking into account the pioneer work by W. Tiller, is an evidence of the role of water as a means of information storage. The water structure varies under the effect of the directed human intention; this can be taken as a first working hypothesis. The second hypothesis is that structurized water affects the condition of space where the water sample is. And, finally, the structurized water affects the state of the organism of the person who drinks it, this is the third, and probably, the most important hypothesis. If we manage to prove these hypotheses experimentally, a new understanding of the mystique of the world will open up before Mankind
 
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